Analysis of pituitary cells by laser capture microdissection.

Genetic analysis of HIV by in situ PCR-directed laser capture microscopy of infected cells.

Behind the exponential expansion of the acquired immunodeficiency syndrome (AIDS) epidemic, there is a continuous and progressive molecular evolution of human immunodeficiency virus (HIV)-1. In this regard, the molecular analysis of viral strains infecting several anatomic compartments in humans has become critical to understanding AIDS-related pathologies and to improving emerging therapeutic ...

Detection of Ki-ras and p53 mutations by laser capture microdissection/PCR/SSCP.

Efficient detection of somatic mutations is important for the development of clinical molecular diagnostic assays. However, the detection of somatic mutations in tissue is confounded by dilution of the tumor cell population by normal cells. Laser microdissection allows enrichment for tumor-associated genetic alterations to take place at the level of cell selection, eliminating the need to enric...

Author's response to reviews Title: Analysis of gene expression in prostate cancer epithelial and interstitial stromal cells using laser capture microdissection Authors:

Application of laser capture microdissection and proteomics in colon cancer.

AIMS Laser capture microdissection is a recent development that enables the isolation of specific cell types for subsequent molecular analysis. This study describes a method for obtaining proteome information from laser capture microdissected tissue using colon cancer as a model. METHODS Laser capture microdissection was performed on toluidine blue stained frozen sections of colon cancer. Tum...

Laser-capture microdissection.

Laser-capture microdissection is a recently discovered state-of-the-art method to obtain cells for genetic analysis. It is a one-step procedure that allows capture of selected cells under direct microscopic visualization.